In September 1992 Darren Knight Gallery opened with a group exhibition at 65 Smith Street, Fitzroy, Melbourne. Exhibiting mainly Australian and New Zealand artists, the gallery operated from this small shop front space for four years before relocating to Sydney.

In February 1997 Darren Knight Gallery Sydney opened in a former pleating factory at 840 Elizabeth Street, Waterloo, the first gallery to establish itself in what is now a thriving arts precinct.